Isolation and characterization of ropA homologous genes from Rhizobium leguminosarum biovars viciae and trifolii
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چکیده
منابع مشابه
Cloning and characterization of hydrogen uptake genes from Rhizobium leguminosarum.
A gene library of genomic DNA from the hydrogen uptake (Hup)-positive strain 128C53 of Rhizobium leguminosarum was constructed by using the broad-host-range mobilizable cosmid vector pLAFR1. The resulting recombinant cosmids contained insert DNA averaging 21 kilobase pairs (kb) in length. Two clones from the above gene library were identified by colony hybridization with DNA sequences from plas...
متن کاملThe Rhizobium Ieguminosarum biovar viciae nod0 gene can enable a nod€ mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
Sciences, University of East Anglia, Norwich NR4 7TJ, UK 2 School of Biological Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute t o nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of ...
متن کاملCharacterization of genes involved in erythritol catabolism in Rhizobium leguminosarum bv. viciae.
A genetic locus encoding erythritol uptake and catabolism genes was identified in Rhizobium leguminosarum bv. viciae, and shown to be plasmid encoded in a wide range of R. leguminosarum strains. A Tn5-B22 mutant (19B-3) unable to grow on erythritol was isolated from a mutant library of R. leguminosarum strain VF39SM. The mutated gene eryF was cloned and partially sequenced, and determined to ha...
متن کاملMolecular characterization of the nodulation gene, nodT, from two biovars of Rhizobium leguminosarum.
DNA sequencing of the nodIJ region from Rhizobium leguminosarum biovar trifolii revealed the nodT gene immediately downstream of nodJ. DNA hybridizations using a nodT-specific probe showed that nodT is present in several R. leguminosarum strains. Interestingly, a flavonoid-inducible nodT gene homologue in R. leguminosarum bv. viciae is not in the nodABCIJ operon but is located downstream of nod...
متن کاملبررسی برخی خصوصیات فیزیولوژیک Rhizobium leguminosarum bv. viciae بومی ایران
نیتروژن یکی از مهمترین و پرنیازترین عناصر برای رشد گیاهان است. نیتروژن در کشاورزی، معمولا از طریق کود شیمیایی در اختیار گیاه قرار می‌گیرد که باعث مشکلات زیست محیطی فراوانی می‌شود.. تثبیت زیستی نیتروژن از پدیده‌های طبیعی و استثنایی است که توسط گروهی از باکتری‌ها از جمله ریزوبیوم‌ها انجام می‌شود. باکتری اختصاصی همزیست تثبیت کننده نیتروژن باقلا Rhizobium leguminosarum bv. viciae می‌باشد. یکی از را...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1995
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.177.17.4985-4991.1995